Set of dATP, dCTP, dGTP, dTTP,
100mM each
Storage
Recommendations: Always avoid freeze-thaw
cycles
or exposure to frequent
temperature changes. These fluctuations
can greatly alter product stability.
Long term (Infrequent use; 1-2 times per month): -70℃
Daily/Weekly use: -20℃
|
2’-Deoxyadenosine-5’-Triphosphate,Sodium |
2’-Deoxycytidine-5’-Triphosphate, |
|
Salt (dATP) |
Sodium Salt (dCTP) |
|
Sequenase and PCR Qualified |
Sequenase and PCR Qualified |
|
C10H12N5O12P3Na4 |
C9H12N3O13P3Na4 |
|
Formula Wt. 579.2 |
Formula Wt. 555.1 |
|
Specifications: |
Specifications: |
|
Form: Aqueous solution, 0.1M |
Form: Aqueous solution, 0.1M |
|
Purity (HPLC): >99% |
Purity (HPLC): >99% |
|
λmax:
259nm+2nm |
λmax:
272nm+2nm |
|
Absorbance rations (pH 7.0): |
Absorbance rations (pH 7.0): |
|
A250/A260 0.80+0.03 |
A250/A260 0.80+0.03 |
|
A280/A260 0.15+0.03 |
A280/A260 0.96+0.03 |
|
PH: 7.5 +0.1 |
PH: 7.5 +0.1 |
|
|
|
|
2’-Deoxyguanosine-5’-Triphosphate, |
2’-Deoxythymidine-5’-Triphosphate, |
|
Sodium Salt (dGTP) |
Sodium Salt (dTTP) |
|
Sequenase and PCR Qualified |
Sequenase and PCR Qualified |
|
C10H12N5O13P3Na4 |
C10H13N2O14P3Na4 |
|
Formula Wt. 595.1 |
Formula Wt. 570.1 |
|
Specifications: |
Specifications: |
|
Form: Aqueous solution, 0.1M |
Form: Aqueous solution, 0.1M |
|
Purity (HPLC): >99% |
Purity (HPLC): >99% |
|
λmax:
253nm+2nm |
λmax:
267nm+2nm |
|
Absorbance rations (pH 7.0): |
Absorbance rations (pH 7.0): |
|
A250/A260 1.15+0.03 |
A250/A260 0.65+0.03 |
|
A280/A260 0.67+0.03 |
A280/A260 0.73+0.03 |
|
PH: 7.5+ 0.1 |
PH: 7.5 +0.1 |
Quality control:
21.
The concentration is verified by optical density spectrophotometry.
22.
This preparation is free of DNase and RNase contamination as determined
by incubation of the 4 dNTPs with radioactive substrates.
23.
The purity of this preparation is determined by HPLC. The minimum passing
specification for the triphosphate content is 99%.
24.
This lot of dNTPs has been performance tested with Sequenase, Taq
DNA polymerase, Pfu DNA polymerase and T7 DNA polymerase.
The
performance of this product is guaranteed for twelve months from the date
of purchase.
|
