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Product：2’-Deoxycytidine-5’-Triphosphate,
Sodium Salt (dCTP)
Storage
Recommendations: Always avoid freeze-thaw
cycles
or exposure to frequent temperature changes. These
fluctuations can greatly alter product stability.
Long term (Infrequent use; 1-2 times per month): -70℃
Daily/Weekly use: -20℃
Specification Sheet:
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2’-Deoxycytidine-5’-Triphosphate,
Sodium Salt (dCTP) |
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Sequenase and PCR Qualified |
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C9H12N3O13P3Na4 |
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Formula Wt. 555.1 |
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Specifications: |
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Form: Aqueous solution, 0.1M |
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Purity (HPLC): >99% |
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λmax:
272nm+2nm |
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Absorbance rations (pH 7.0): |
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A250/A260 0.80+0.03 |
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A280/A260 0.96+0.03 |
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PH: 7.5 +0.1 |
Quality control:
1.
The concentration is verified by optical density spectrophotometry.
2.
This preparation is free of DNase and RNase contamination as determined
by incubation of the 4 dNTPs with radioactive substrates.
3.
The purity of this preparation is determined by HPLC. The minimum passing
specification for the triphosphate content is 99%.
4.
This lot of dNTPs has been performance tested with Sequenase, Taq
DNA polymerase, Pfu DNA polymerase and T7 DNA polymerase.
The
performance of this product is guaranteed for twelve months from the date
of purchase.
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