Specification Sheet (dTTP)

 

Product : 2' -Deoxythymidine -5' -Triphosphate, Sodium Salt (dTTP)

Storage Recommendations: Always avoid freeze-thaw cycles or exposure to frequent temperature changes. These fluctuations can greatly alter product stability.

Long term (Infrequent use; 1-2 times per month): -70oC

Daily/Weekly use: -20oC

 

Product :

2' -Deoxythymidine -5' -Triphosphate, Sodium Salt (dTTP)

Sequenase and PCR Qualified

Molecular Formula:

C10H13N2O14P3Na4

Molecular Weight:

570.1

Form:

Aqueous solution, 100mM

Purity (HPLC):

>99%

?max:

267nm ± 2nm

Absorbance rations (pH 7.0):

A250/A260 0.65 ± 0.03

 

A280/A260 0.73 ± 0.03

PH:

7.5 ± 0.1

Quality control:

1.   The concentration is verified by optical density spectrophotometry.

2.   This preparation is free of DNase and RNase contamination as determined by incubation of the 4 dNTPs with radioactive substrates.

3.   The purity of this preparation is determined by HPLC. The minimum passing specification for the triphosphate content is 99%.

4.   This lot of dNTPs has been performance tested with Sequenase, Taq DNA polymerase, Pfu DNA polymerase and T7 DNA polymerase.

 

The performance of this product is guaranteed for twelve months from the date of purchase.

 


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