| Description
Pfu DNA Polymerase is a thermostable enzyme of approximately 92kDa isolated from Pyrococcus furiosus (1). The enzyme replicates DNA at 7 5 ? , catalyzing the polymerization of nucleotides into duplex DNA in the 5' ? 3' direction in the presence of magnesium. Pfu DNA Polymerase also possesses 3' ? 5' exonuclease (proofreading) activity. Base misinsertions that may occur during polymerization are rapidly excised by the proofreading activity of the polymerase. Consequently, Pfu DNA Polymerase is recommended for use in PCR and primer extension reactions that require highfidelity synthesis (2-5). Pfu DNA Polymerase generated PCR fragments are blunt-ended.
Quality Control
1. Standard DNA Polymerase Assay Conditions
2. Endonuclease Assay
3. Exonuclease Assay
4. Functional Assay Features
High Fidelity: Pfu DNA Polymerase exhibits the lowest error rate of any thermostable DNA polymerase.
Complete: Provided with 10 × Buffer containing 20 mM MgSO4.
Applications
Pfu DNA Polymerase is recommended for use in PCR, primer extension reactions and other applications that demand high fidelity.
References
• Fiala, G. and Stetter, K.O. (1986) Arch.Microbiol. 145 , 56.
• Lundberg, K.S. et al. (1991) Gene. 108 , 1-6.
• Flaman, J.M. et al. (1994) Nucl. Acids Res. 22 , 3259-60.
• Cline, J. et al. (1996) Nucl. Acids Res. 24 , 3546-51.
• Andre, P. et al. (1997) Genome Res. 7 , 843-52.
Order Information
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