| Description
Natural Ribonuclease (RNase) Inhibitor has broad spectrum RNase inhibitory properties, including the inhibition of eukaryotic RNases of the neutral type (1). The 50kDa protein exerts its inhibitory effect by noncovalently binding to RNases in a 1:1 ratio. The K i value for the binding of RNasin Ribonuclease Inhibitor to RNase (e.g., RNase A) is approximately10 -14 M (2-4).
Features
Inhibits Common Eukaryotic RNases: RNasin Ribonuclease Inhibitor has broad-spectrum RNase inhibitory properties, including RNase A, RNase B, RNase C and human placental RNase. Does no inhibit RNase T1, S1 nuclease, RNase from Aspergillus, RNase H.
Compatibility: RNasin Ribonuclease Inhibitor does not inhibit SP6, T7 or T3 RNA Polymerase; AMV or M-MLV Reverse Transcriptase; or Taq DNA Polymerase.
Broad pH Range: Active over a broad pH range (pH 5-8).
Applications • RT-PCR.
• Useful in any applications where eukaryotic RNase contamination is a potential problem.
• Protection of mRNA in cDNA synthesis reactions.
• In vitro transcription/translation.
• Increase yield and activity of polysomes.
• Improvement of in vitro virus replication.
• Improvement of RNA translation in homologous systems.
• Preparation of RNase-free antibody.
• Enhancement of RNA yields from Riboprobe System RNA synthesis reactions.
References
1.Blackburn, P. and Moore , S. (1982) In: The Enzymes, Vol. XV, Part B.
2.Blackburn , P. et al. (1977) J. Biol. Chem. 252 , 5904-10.
3.Lee, F.S. et al. (1989) Biochemistry 28 , 219-24.
4.Lee, F.S. et al. (1989) Biochemistry 28 , 225-30.
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