| Description
S1 Nuclease degrades single-stranded DNA and RNA endonucleolytically to yield 5' -phosphoryl terminated products. Double-stranded nucleic acids (DNA:DNA, DAN:RNA or RNA:RNA) are resistant to degradation except with extremely high concentrations of enzyme(1). The enzyme is used to remove single-stranded termini from double-stranded DNA(2), for selective cleavage of singl-stranded DNA and for mapping RNA transcripts(3).
Quality Control
Contaminant Activity Assay
S1 Nuclease (100 units) is incubated with 1mg Lambda DNA/Hind III Markers for 10 minutes at 25¡ãC . A sharp banding pattern of the DNA Marker will result when the samples are electrophoresed and visualized on an ethidium bromide-stained agarose gel. Applications
1.Removal of protruding single-stranded termini in double-stranded DNA(2).
2. Selective cleavage of single-stranded DNA.
3. Mapping RNA transcripts(3).
References
1. Vogt, V.M. (1973) Eur. J. Biochem. 33, 192-200.
2. Roberts, T.M. et al. (1979) Proc. Natl. Acad. Sci. USA 76, 760-4.
3. Berk, A.J. and Sharp, P.A. (1978) Proc. Natl. Acad. Sci. USA 75, 1274-8.
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