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M-MLV Reverse Transcriptase, RNase H Minus

M-MLV Reverse Transcriptase, RNase H Minus
Catalog No.
ON-047
Purity
>95% by SDS-PAGE
Storage
-20℃
Source
Recombinant E.coli

Product description: M-MLV Reverse Transcriptase, RNase H Minus is an RNA-dependent DNA polymerase with no detectable RNase H activity. A point mutation in the RNase H domain increases the thermostablity of the enzyme and support greater cDNA yield of full-length transcripts than wild type M-MLV Reverse Transcriptase.

Source: Recombinant E.coli

Concentration and Size: 200U/μL

Unit Definition: One unit is defined as the amount of enzyme required to catalyze the transfer of 1nmol of deoxynucleotides into acid-precipitable material in 10 minutes at 37℃.

Storage Buffer: 20mM Tris-HCl (pH7.5 at 25℃), 0.1mM EDTA, 200mM NaCl, 0.01% NP-40, 1mM DTT and 50% Glycerol.

Companion Product: 5X RT Reaction Buffer, Cat#ON-067, 250mM Tris-HCl (pH8.3 at 25℃),375mM KCl, 15mM MgCl2, 50mM DTT.

1. Before the first-strand Synthesis of DNA, heat the primer(about 0.5μg) and template (up to 2μg) to 70℃ for 5min to melt secondary structure within the template. Cool the tube on ice to prevent secondary structure from reforming.

2. Add the following components to the annealed primer/template:



3. Incubate for 60min at 42℃.

Storage Conditions: -20℃

Quality Assurance: Free of endonuclease, exonuclease, Nickase and RNase activities.

Physical Purity: >95% by SDS-PAGE.

1. Yu Chen, Weiguo Xu,Qining Sun, Biotechnol Lett. 31, 1051–1057 (2009).

2. Shufeng Liu, Stephen P. Goff, et al. FEBS Letters. 580, 1497-1501(2006).

3. Monica J. Roth, Naoko Tanese, and Stephen P. Goff, The Journal Of Biological Chemistry. 260, 9326-9335(1985).