Product description: DNase I (RNase free) is an endonuclease that digests single- and double-stranded DNA. It nonspecifically cleaves DNA to release di-,tri- and oligonucleotide products with 5'-phosphorylated and 3'-hydroxylated end.

Source: Recombinant E.coli

Concentration and Size: 1U/μL

Unit Definition: One unit is defined as the amount of enzyme required to completely degrade 1μg of λDNA in 10 minutes at 37℃.

Storage Buffer: 50mM Tris-HCl (pH7.5, 25℃), 10mM CaCl₂ and 50% Glyercol

Companion Product: 10X DNase I Reaction Buffer, Cat#ON-077; 100mM Tris-HCl pH7.6, 25℃, 25mM MgCl₂, 5mM CaCl₂

Related Products:

High Yield T7 RNA Synthesis Kit, Cat#ON-040

T7 RNA polymerase, Cat#ON-004

10X DNase I Reaction Buffer, Cat#ON-077

DNase I treatment after IVT reaction:

1. Directly add DNase I (1unit DNase I per ug of DNA template) to IVT Reaction, mix well and incubate 30 min at 37°C.

2. Add 0.5M EDTA to a final concertation over one millimole than divalent cations.

3. Heat inactivate at 75℃ for 10min.

Storage Conditions: -20℃

Quality Assurance: Free of RNase activities.

Physical Purity: ＞95% by SDS-PAGE.

1. A F Worrall，B A Connolly, J，Biol，Chem. 265,21889–21896 (1990).

2. Ching-Ying Chen，Shao-Chun Lu，et al. Gene. 206, 181-184(1998).