Product description: mRNA Cap-2'-O-Methyltransferase is a recombinant protein from Vaccinia virus. The enzyme can add a methyl group at the 2'-O position of the first nucleotide adjacent to the Cap structure at the 5' end of the RNA. The enzyme utilizes SAM as a methyl donor to methylate capped RNA (Cap 0) resulting in a cap-1 structure. The Cap-1structure has been reported to enhance mRNA translation efficiency and hence may help improve expression in mRNA transfection and microinjection experiments. mRNA cap-2'-O-Methyltransferase specifically requires RNA with an m7GpppN Cap as substrate.
Source: Recombinant E.coli
Concentration and Size: 50U/μL
Unit Definition: One unit is defined as the amount of enzyme required to methylate 10pmols of 80 nt Capped RNA transcript in 1 hour at 37℃.
Storage Buffer: 20mM Tris-HCl (pH8.0), 0.1mM EDTA, 100mM NaCl, 1mM DTT, 0.1%(v/v) Triton X-100, 50%(v/v) glycerol.
Companion Product: 10X Capping Buffer, Cat#ON-073, 500mM Tris-HCl, pH8.0, 50mM KCl, 10mM MgCl2, 10mM DTT; S-adenosylmethionine (SAM),32mM, Cat#ON-074.
Storage Conditions: -20℃
Quality Assurance: Free of Endonuclease, Exonuclease, and RNase activities.
Physical Purity: ＞95% by SDS-PAGE.
