Product description: Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5'→ 3' polymerase activity and 5'→ 3' exonuclease activity. The PCR product can be cloned directly into a T-vector (TA cloning) due to the addition of an adenosine(A) to the 3' end. The thermostable enzyme is a single polypeptide chain with a molecular weight of approximately 94kD.
Source: Recombinant E.coli
Concentration and Size: 5U/μL
Unit Definition: One unit is defined as the amount of enzyme that incorporates 10nmols of total dNTPs into acid-insoluble DNA in 30min at 74℃.
Storage Buffer: 20mM Tris-HCl (pH7.9, 25℃), 0.1mM EDTA, 100mM KCl, 0.5% Tween20, 0.5% NP40, 1mM DTT, 50%(v/v) Glycerol.
Companion Products: 10X Reaction Buffer, Cat#ON-012, 100mM Tris-HCl, 500mM KCl, pH9.0, 25℃; 100mM MgCl2, Cat#ON-013.
1. Recommended reaction mixture:
2. Recommended reaction conditions:
Optimal enzyme concentration: 0.5U-5U per 50μL volume (50μL volume: 1.25U recommended)
Optimal MgCl2 concentration: 1.5mM (1~5mM)
Storage Conditions: -20℃
Quality Assurance: Free of Endonuclease, Exonuclease, Nickase and RNase activities. Free of 16s rDNA.
Physical Purity: ＞95% by SDS-PAGE.
