Product description: PPase is prepared from an E. coli strain containing Saccharomyces cerevisiae ppa gene. It is a homodimer consisting of two equal subunits of molecular weight 32-35 kDa. The enzyme catalyzes the hydrolysis of inorganic pyrophosphate into two orthophosphates.
Source: Recombinant E.coli
Concentration and Size: 0.1U/μL
Unit Definition: One unit is the amount of enzyme that will generate 1 μmol of phosphate per minute from inorganic pyrophosphate under standard reaction conditions (a 10 minute reaction at 25℃ in 100mM Tris-HCl, [pH 7.2], 2mM MgCl2 and 2mM PPi in a reaction volume of 0.5 mL)
Storage Buffer: 20 mM Tris-HCl PH8.0, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol.
Storage Conditions: -20℃
Quality Assurance: Free of endonuclease, exonuclease and RNase activities.
Physical Purity: ＞95% by SDS-PAGE.
