Enzymes

 

Enzymes are biological catalysts that accelerate specific chemical reactions in a cell. In molecular diagnostics, they are used in various applications such as:

 

Nucleic Acid Amplification: Enzymes like DNA polymerases are crucial for techniques like PCR (Polymerase Chain Reaction), which amplifies specific DNA sequences for detection.

 

Restriction Digestion: Restriction enzymes cleave DNA at specific recognition sequences, creating fragments used for analysis in techniques like gel electrophoresis and Southern blotting.

 

Ligation: Ligases join DNA fragments, used in cloning and DNA modification techniques.

 

Probe Digestion: Enzymes can digest probes used in diagnostic assays after they hybridize to their target molecules.

 

Buffers

 

Buffers are solutions that maintain a constant pH in a reaction. They are essential in molecular diagnostics because many enzymes have specific pH ranges for optimal activity. Buffers also help to maintain ionic strength and prevent unwanted interactions between biomolecules. Here's how buffers are important in diagnostics:

 

Maintaining Enzyme Activity: Enzymes function best within a specific pH range. Buffers ensure this optimal pH is maintained throughout the diagnostic assay.

 

Optimizing Reaction Conditions: Buffers can influence factors like ionic strength, which can affect how biomolecules interact and how efficiently reactions proceed.

 

Standardization: Buffers with consistent compositions allow for standardized diagnostic protocols, ensuring reliable and reproducible results.

 


In conclusion, enzymes and buffers are essential tools in molecular biological diagnostics. Enzymes act as biological catalysts to drive specific reactions, while buffers provide optimal reaction conditions by maintaining pH and ionic strength. Their combined application allows for the sensitive and specific detection of diseases, genetic variations, and other biological targets.

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カタログダウンロード

合計製品数 0

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pfu DNA polymerase

カタログ番号: ON-051
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10X IVT Reaction Buffer without Mg2+

カタログ番号: ON-041
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100mM MgCl2

カタログ番号: ON-013
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10X Cut Buffer without BSA

カタログ番号: ON-268
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10X T4 RNA ligase 1 Reaction Buffer

カタログ番号: ON-566
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10X MnCl2

カタログ番号: ON-079
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10X Cut Buffer II without BSA

カタログ番号: ON-226
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10mM GTP

カタログ番号: ON-075
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50% PEG 8000

カタログ番号: ON-112
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10X T4 RNA ligase 2 Reaction Buffer

カタログ番号: ON-511
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10X RNaseR Reaction Buffer

カタログ番号: ON-510
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S-adenosylmethionine (SAM),32mM

カタログ番号: ON-074
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25mM MgCl2

カタログ番号: ON-060
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10X Taq DNA Ligase Reaction Buffer

カタログ番号: ON-072
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10X T4 DNA Ligase Reaction Buffer

カタログ番号: ON-158
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10X EDTA

カタログ番号: ON-080
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10X RNase III Reaction Buffer

カタログ番号: ON-078
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10X Phosphatase Reaction Buffer

カタログ番号: ON-180
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5×RT Reaction Buffer

カタログ番号: ON-067
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10X Reaction Buffer for Taq

カタログ番号: ON-012
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10X poly(A) Polymerase Reaction Buffer

カタログ番号: ON-127
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10X DNase I Reaction Buffer

カタログ番号: ON-077
enzymes_img2.svg

10X pfu Reaction Buffer

カタログ番号: ON-068
enzymes_img2.svg

10X IVT Reaction Buffer

カタログ番号: ON-062
enzymes_img2.svg

10X Capping Buffer

カタログ番号: ON-073
enzymes_img1.svg

RNase III, E.coli

カタログ番号: ON-024
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Capped RNA Synthesis Kit

カタログ番号: ON-257
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Ribonuclease Inhibitor, Human Placenta

カタログ番号: ON-039
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High Yield T7 RNA Synthesis Kit

カタログ番号: ON-040
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T4 RNA Ligase 1

カタログ番号: ON-081
enzymes_img1.svg

DNase I (RNase-free)

カタログ番号: ON-109
enzymes_img1.svg

T4 RNA Ligase 2

カタログ番号: ON-544-C010
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Alkaline Phosphatase, TAB5

カタログ番号: ON-179
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RNase R

カタログ番号: ON-509-C020
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mRNA Cap-2'-O-Methyltransferase

カタログ番号: ON-014
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Pyrophosphatase Inorganic (yeast)

カタログ番号: ON-025
enzymes_img1.svg

Vaccinia Capping Enzyme

カタログ番号: ON-028
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Uracil-DNA Glycosylase(UNG), E.coli

カタログ番号: ON-056
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Taq DNA Ligase

カタログ番号: ON-308
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BspQI

カタログ番号: ON-124
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T4 DNA Ligase

カタログ番号: ON-157
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Taq DNA polymerase

カタログ番号: ON-007
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T7 RNA polymerase

カタログ番号: ON-004
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Poly(A) Polymerase

カタログ番号: ON-126
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BspQI (10U/ul)

製品仕様: GMP
カタログ番号: ON-124-G
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T7 RNA polymerase (50U/ul)

製品仕様: GMP
カタログ番号: ON-004-G
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T7 RNA polymerase (200U/ul)

製品仕様: GMP
カタログ番号: ON-004-C200-G
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DNase I (RNase-free) (1U/ul)

製品仕様: GMP
カタログ番号: ON-109-G
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Pyrophosphatase Inorganic (yeast) (0.1U/ul)

製品仕様: GMP
カタログ番号: ON-025-G
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Vaccinia Capping Enzyme

製品仕様: GMP
カタログ番号: ON-028-G
enzymes_img1.svg

2'-O-Methyltransferase (50U/ul)

製品仕様: GMP
カタログ番号: ON-014-G
enzymes_img1.svg

RNase Inhibitor (40U/ul)

製品仕様: GMP
カタログ番号: ON-039-G
enzymes_img2.svg

10X IVT Reaction Buffer

製品仕様: GMP
カタログ番号: ON-062-G
enzymes_img2.svg

10X Capping Buffer

製品仕様: GMP
カタログ番号: ON-073-G
enzymes_img2.svg

10X Cut Buffer

製品仕様: GMP
カタログ番号: ON-268-G
enzymes_img2.svg

S-adenosylmethionine (SAM)

製品仕様: GMP
カタログ番号: ON-074-G