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Pyrophosphatase Inorganic (yeast)

  • Cat.No.ON-025
  • Cas.No.
  • SourceRecombinant E.coli
  • Purity>95% by SDS-PAGE
  • Storage-20℃
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Product description: PPase is prepared from an E. coli strain containing Saccharomyces cerevisiae ppa gene. It is a homodimer consisting of two equal subunits of molecular weight 32-35 kDa. The enzyme  catalyzes the hydrolysis of inorganic pyrophosphate into two orthophosphates.

Source: Recombinant E.coli

Concentration and Size: 0.1U/μL

Unit Definition: One unit is the amount of enzyme that will generate 1 μmol of phosphate per minute from inorganic pyrophosphate under standard reaction conditions (a 10 minute reaction at 25℃ in 100 mM Tris-HCl, [pH 7.2], 2 mM MgCl2 and 2 mM PPi in a reaction volume of 0.5 mL)

Storage Buffer: 20 mM Tris-HCl PH8.0, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol

Related Products:

High Yield T7 RNA Synthesis Kit, Cat#ON-040

T7 RNA polymerase, Cat#ON-004

Poly(A) Polymerase, Cat#ON-126

Alkaline Phosphatase,TAB5, Cat#ON-179

When in vitro transcription, higher yields of RNA may be obtained by adding inorganic pyrophosphatase to a final concentration of 4 units/mL.

Storage Conditions: -20℃

Quality Assurance: Free of endonuclease, exonuclease and RNase activities.

Physical Purity: >95% by SDS-PAGE.

1. Cooperman, B.S., The mechanism of action of yeast inorganic pyrophosphatase, Meth. Enzymol., 87, 526-548, 1982.

2. Cunningham, P.R. and Ofengand, J., Use of inorganic pyrophosphatase to improve the yield of in vitro transcription reactions catalyzed by T7 RNA polymerase, Biotechniques, 9, 713-714, 1990.

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