Product description: PPase is prepared from an E. coli strain containing Saccharomyces cerevisiae ppa gene. It is a homodimer consisting of two equal subunits of molecular weight 32-35 kDa. The enzyme catalyzes the hydrolysis of inorganic pyrophosphate into two orthophosphates.
Source: Recombinant E.coli
Concentration and Size: 0.1U/μL
Unit Definition: One unit is the amount of enzyme that will generate 1 μmol of phosphate per minute from inorganic pyrophosphate under standard reaction conditions (a 10 minute reaction at 25℃ in 100 mM Tris-HCl, [pH 7.2], 2 mM MgCl2 and 2 mM PPi in a reaction volume of 0.5 mL)
Storage Buffer: 20 mM Tris-HCl PH8.0, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol
High Yield T7 RNA Synthesis Kit, Cat#ON-040
Poly(A) Polymerase, Cat#ON-126
When in vitro transcription, higher yields of RNA may be obtained by adding inorganic pyrophosphatase to a final concentration of 4 units/mL.
Storage Conditions: -20℃
Quality Assurance: Free of endonuclease, exonuclease and RNase activities.
Physical Purity: ＞95% by SDS-PAGE.
1. Cooperman, B.S., The mechanism of action of yeast inorganic pyrophosphatase, Meth. Enzymol., 87, 526-548, 1982.
2. Cunningham, P.R. and Ofengand, J., Use of inorganic pyrophosphatase to improve the yield of in vitro transcription reactions catalyzed by T7 RNA polymerase, Biotechniques, 9, 713-714, 1990.