Product description: The High Yield T7 RNA Synthesis Kit is designed to produce 25-fold more full-length RNA transcript per reaction than conventional in vitro transcription reactions. The yield is up to 210μg RNA per reaction.
Materials provided with the kit:
The kit contains sufficient reagents for 50 reaction of 20μL each.
Note: 5X Reaction Buffer store at -70℃ may result in the formation of a white precipitate. If this happens, heat the tube to 37℃ for 5 minutes and mix thoroughly to resuspend the precipitate.
1. The following amounts are for a single 20 μL reaction. Reactions may be scaled up or down if desired. 
*The transcript may vary depending on the amount of template DNA.
2. Mix thoroughly and incubate at 37℃ 2h.
3. (optional)Add 1μL DNase I (RNase-free), mix well and incubate 30 min at 37°C.
Storage Conditions: -20℃
Quality Assurance: All components are tested in a functional assay as described in this procedure. A 20μL reaction containing 1 μg of the control template DNA which codes for a ~800b transcript synthesized >150μg of RNA after a 2 hr incubation.
