Product description: Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5'→ 3' polymerase activity and 5'→ 3' exonuclease activity. The PCR product can be cloned directly into a T-vector(TA cloning) due to the addition of an adenosine(A) to the 3' end. The thermostable enzyme is a single polypeptide chain with a molecular weight of approximately 94kD.
Source: Recombinant E.coli
Concentration and Size: 5U/μL
Unit Definition: One unit is defined as the amount of enzyme that incorporates 10nmols of total dNTPs into acid-insoluble DNA in 30min at 74℃.
Storage Buffer: 20mM Tris-HCl (pH7.9, 25℃), 0.1mM EDTA, 100mM KCl, 0.5% Tween20, 0.5% NP40, 1mM DTT, 50%(v/v) Glycerol.
Companion Products: 10X Reaction Buffer, Cat#ON-012, 100mM Tris-HCl, 500mM KCl, pH9.0, 25℃; 100mM MgCl2, Cat#ON-013.
Related Products:
pfu DNA polymerase, Cat#ON-051
Uracil-DNA Glycosylase (UNG), heat-labile, Cat#ON-054
Uracil-DNA Glycosylase(UNG), E.coli, Cat#ON-056
1. Recommended reaction mixture:
| Final Volume | Final conc | |
| 10X Reaction buffer | 5μL | 1X |
| dNTP Mix, 10mM each | 1 | 0.2mM each |
| 25mM MgCl2 | 3 | 1.5mM |
| upstream primer | X μL | 0.1-1.0μM |
| downstream primer | Y μL | 0.1-1.0μM |
| Taq (5U/μl) | 0.25μL | 1.25U |
| Template DNA | Z μL | <0.5μg/50μL |
| Nuclease-Free H2O | to 50μL |
2. Recommended reaction conditions:
| Cycle | Time | Temp ℃ | |
| Initial Denaturation | 1 | 2min | 95 |
| Denaturation | 0.5-1min | 94 | |
| Annealing | 25-40 | 0.5-1min | Tm-5 |
| Extension | 1min/kb | 72 | |
| Final extension | 1 | 5min | 72 |
Optimal enzyme concentration: 0.5U-5U per 50μL volume (50μL volume: 1.25U recommended)
Optimal MgCl2 concentration: 1.5mM (1~5mM)
Storage Conditions: -20℃
Quality Assurance: Free of Endonuclease, Exonuclease, Nickase and RNase activities.
Free of 16s rDNA.
Physical Purity: ＞95% by SDS-PAGE.
1. Lawyer FC,et al. PCR Methods Appl. 2(4):275-87(1993).
2. Chien A, Edgar DB, Trela JM.J. Bacteriol 127(3):1550-7(1976).