PMO monomers, specifically phosphorodiamidate morpholino (PDM) monomers, play a vital role in the synthesis of oligonucleotides called phosphorodiamidate morpholino oligonucleotides (PMOs). PMOs are a class of antisense therapeutic agents that bind to target RNA molecules and modulate gene expression. Here's a breakdown of how PMO monomers are applied in oligo synthesis:



• PMO monomers act as the building blocks for PMO oligonucleotides.

• Each PMO monomer contains a morpholino ring, a linker group, and a phosphoramidate moiety.

• The morpholino ring specifically recognizes and binds to complementary RNA sequences.

• The linker group connects the morpholino ring to the phosphoramidate moiety.

• The phosphoramidate moiety plays a crucial role in linking individual PMO monomers during oligo synthesis.



• PMO synthesis typically employs a solid-phase approach similar to DNA synthesis.

• A controlled sequence of PMO monomers is attached to a solid support one by one.

• Techniques like Fmoc (Fluorenylmethoxycarbonyl) or trityl chemistry are used to protect and deprotect functional groups on the monomers during the coupling process.

• After chain assembly, the PMO oligonucleotide is cleaved from the solid support and deprotected to obtain the final product.


Advantages of PMO Monomers:

Compared to traditional DNA oligonucleotides, PMO oligonucleotides made with PMO monomers offer several advantages:

• Increased stability: PMO backbone is more resistant to enzymatic degradation by nucleases.

• Improved binding affinity: PMO oligonucleotides can exhibit higher binding affinity towards target RNA.

• Reduced toxicity: PMO oligonucleotides generally demonstrate lower off-target effects and toxicity compared to DNA counterparts.


Challenges and Future Directions:

Although PMO monomers offer numerous benefits, there are ongoing efforts to improve PMO synthesis:

• Optimizing deprotection and coupling methods for higher efficiency.

• Developing new PMO chemistries compatible with automated synthesizers for faster and more scalable production.

• Creating new PMO conjugates with improved delivery properties.


Overall, PMO monomers are a critical component in the synthesis of PMO oligonucleotides, a promising class of therapeutic agents with the potential to treat various diseases.



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Morpholino A subunit

Catalog No.: PR1-007
Molecular Formula: C38H37ClN7O4P
CAS No.: 956139-18-7

Morpholino C subunit

Catalog No.: PR3-014
Molecular Formula: C37H37ClN5O5P
CAS No.: 956139-21-2

Morpholino T subunit

Catalog No.: PR4-003
Molecular Formula: C31H34ClN4O5P
CAS No.: 956139-30-3

O(6)-NPE-N(2)-iBu protected G PMO Monomer

Catalog No.: PR2-012
Molecular Formula: C43H46ClN8O7P

Morpholino U

Catalog No.: PR5-074
Molecular Formula: C30H32ClN4O5P

Morpholino G(PhAc) subunit

Catalog No.: PR2-051
Molecular Formula: C39H39ClN7O5P

DMO-4AG-N,N-dimethyl phosphoramidochloridate

Catalog No.: OP-202
Molecular Formula: C62H68ClN15O11P2

Morpholino A(Ceoc) subunit

Catalog No.: PR1-113
Molecular Formula: C35H36ClN8O5P

Morpholino C(Ceoc) subunit

Catalog No.: PR3-128
Molecular Formula: C34H36ClN6O6P

O(6)-NPE I PMO Monomer

Catalog No.: OP-260
Molecular Formula: C39H39ClN7O6P