Dyes and quenchers are two essential components in many diagnostic assays, particularly those that utilize fluorescence resonance energy transfer (FRET). FRET is a process where the energy absorbed by a fluorophore (dye) is transferred to a quencher molecule in close proximity. This transfer of energy disrupts the fluorophore's ability to emit its own fluorescence, resulting in a signal that can be measured.

 

Dyes and quenchers are widely used in various diagnostic assays, including:

 

Real-time PCR (qPCR): qPCR is a technique used to detect and quantify specific DNA sequences. In qPCR assays, dyes and quenchers are often attached to oligonucleotide probes that bind to the target DNA sequence. When the probe is intact, the quencher absorbs the energy from the dye, preventing fluorescence. However, when the probe hybridizes to the target DNA, the dye and quencher are separated, allowing the dye to fluoresce. The increase in fluorescence signal indicates the presence and quantity of the target DNA.

 

Immunofluorescence assays (IFAs): IFAs are used to detect antigens (molecules that the body's immune system recognizes) in tissues or cells. In IFAs, dyes can be conjugated to antibodies that bind to specific antigens. The presence of a fluorescent signal indicates the presence of the target antigen.

 

The use of dyes and quenchers allows for the development of highly sensitive and specific diagnostic assays. These assays play a critical role in the diagnosis and management of various diseases.

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OP-011.svg

HEX Dye Amidite

カタログ番号: OP-011
分子式: C46H52N3O10Cl6P
ON-023.svg

5'-Biotin Phosphoramidite

カタログ番号: ON-023
分子式: C46H64N5O6PS
PD4-006.svg

Fluorescein-dT Phosphoramidite

カタログ番号: PD4-006
分子式: C79H89N6O17P
OP-038

Cyanine 3 Phosphoramidite

カタログ番号: OP-038
分子式: C58H70ClN4O4P
OP-028.svg

HQ-F554 Phosphoramidite

カタログ番号: OP-028
分子式: C52H59Cl3N3O10P
OP-057.svg

Cyanine 5 mono MMT phosphoramidite

カタログ番号: OP-057
分子式: C60H72ClN4O4P
ON-101.svg

6-ROX NHS Ester

カタログ番号: ON-101
分子式: C37H33N3O7
ON-088.svg

HDQ-1 CPG 1000Å

カタログ番号: ON-088
ON-095.svg

HDQ-2 CPG 1000Å

カタログ番号: ON-095